Smoothing membrane protein structure determination by initial upstream stage improvements
نویسندگان
چکیده
منابع مشابه
Membrane protein structure determination by electron crystallography.
During the past year, electron crystallography of membrane proteins has provided structural insights into the mechanism of several different transporters and into their interactions with lipid molecules within the bilayer. From a technical perspective there have been important advances in high-throughput screening of crystallization trials and in automated imaging of membrane crystals with the ...
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In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozy...
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Obtaining enough experimental restraints can be a limiting factor in the NMR structure determination of larger proteins. This is particularly the case for large assemblies such as membrane proteins that have been solubilized in a membrane-mimicking environment. Whilst in such cases extensive deuteration strategies are regularly utilised with the aim to improve the spectral quality, these scheme...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2019
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-019-09873-1